Please! Where?! where whould i draw the breakthrough glyph??? i have looked at all of the topics like this and the only answer everybody gets is "you shall be able to draw on it like a tree" and "oh youll see". PLEASE! I HAVE BEEN TRYING FOR HOURS I CANT FIND IT JUST GIVE ME DIRECTIONS OK? and btw do you need all hearts? becuase i killed all brothers but i stull only have 20 hearts and i cant find any more...

Writing in all caps and using poor grammar can make your post very difficult to read :/

Now, in order to draw Breakthrough, you will need to have collected all 20 hearts, filled them completely with color (ie, reached Turgor. Your health bar will begin to flash when you are COMPLETELY full), and unlocked all four hearts on the sister you intend to ascend. What you're reading about "the tree" is how to ascend Sister Death; should you choose to do so, you would draw Breakthrough on her tree. For the other Sisters, merely walk up to them, touch them with a drop of color, and draw Breakthrough as if you were casting Donor.

Ok, but i cannot seem to find the last heart, you see i killed all brothers and when i killed the last one called "Tyrant" i did not recieve a heart. I have looked at walkthroughs and heart locations on forums
but when i go to a location where there is supposed to be a heart, like Unas place, there is no heart there. Even though i have never collected it before. HELP ME!

In summary, I have 20 hearts including the heart of breakthtough, and i am unable to find the last heart.

The last heart is in Montgolfiere, he'll confront you automatically on cycle 30.